Journal: BMC infectious diseases

Article Title: Infectious agents is a risk factor for myxomatous mitral valve degeneration: A case control study.

doi: 10.1186/s12879-017-2387-8

Figure Lengend Snippet: Fig. 4 Bacterial analysis by IHC, ISH and TEM. a: Counts of Mycoplasma pneumoniae (Mp), Chlamydophila pneumoniae (Cp) and Borrelia burgdorferi (Bb) antigens. b, d, f, h, j and l: Normal mitral valve. c, e, g, i, k and m: Mitral valve with myxomatous degeneration; b and c: IHC for Mp; d and e: Mp antigen identification (yellow dots) by Aperio analysis; f and g: IHC for Cp; h and i: Cp antigen identification (yellow dots) by Aperio analysis; j and k: IHC for Bb; l and m: Bp antigen identification (yellow dots) by Aperio analysis. n, o, p and q: Transmission electron microscopy of bacterial elements (arrows). N: A microorganism with Mycoplasma characteristics was identified in the control group, with a long, single envelope membrane and a structure similar to a spirochete in the cytoplasm amid fragmented collagen tissue. O: This structure is consistent with Borrelia, long and winding, and was identified in the control group. P: Structures compatible with Mycoplasma were also identified in the MD group. Q: A microorganism with a wavy structure compatible with spirochete-containing organelles or endosymbionts (consistent with Borrelia) was observed in the MD group. IHC: immunohistochemistry; ISH: in situ hybridization; TEM: transmission electron microscopy; Mp: Mycoplasma pneumoniae; Bb: Borrelia burgdorferi; G1: group 1; G2: group 2; μm2: square micrometers; Ag: antigen; mm: millimeters. Bar =50 μm for B-M. Bar =1 μm for N-Q

Article Snippet: To block endogenous peroxidase, the tissue sections were saturated with 6% hydrogen peroxide for 8 min, followed by avidinbiotin blocking for 20 min, incubation with a protein block for 30 min (Dako, Carpinteria, CA, USA), and then with primary antibodies against Mycoplasma pneumoniae (1:100; rabbit clone 10MR54; Fitzgerald International Inc., Concord, MA, USA), Borrelia burgdorferi (1:300; rabbit clone ab34970, ABCAM), MMP-9 (1:3200; rabbit clone RB1539P; Neo Markers Inc., Fremont, CA, USA), CD20 (1:1000; clone L26 M0755; Dako, Carpinteria, CA, USA), and CD45 (1:125; clone VCHL M0742 Dako, CA, California, USA) overnight.

Techniques: Transmission Assay, Electron Microscopy, Control, Membrane, Immunohistochemistry, In Situ Hybridization

Journal: Scientific Reports

Article Title: Graphene oxide/Cu–MOF-based electrochemical immunosensor for the simultaneous detection of Mycoplasma pneumoniae and Legionella pneumophila antigens in water

doi: 10.1038/s41598-024-68231-y

Figure Lengend Snippet: Illustration of ( A ) the synthesis method used for the preparation of the GO/Cu–MOF composite. ( B ) The steps for the fabrication of the immunosensor and ( C ) the dual detection of M. pneumoniae and L. pneumophila antigens using differential pulse voltammetry.

Article Snippet: Mouse anti- Mycoplasma pneumoniae P1 (6537), mouse anti- Legionella pneumophila (LN29) antibodies, Legionella pneumophila , mycoplasma pneumoniae antigens and interferents such as canine adenovirus 1 (CAV1), human metapneumovirus (hMPV) B, Influenza A (Cambodia) virus (IAV), Influenza B (Austria) virus (IBV), and respiratory syncytial virus B (RSVB) Lysate antigens were purchased from the Native Antigen company, United Kingdom.

Techniques:

Journal: Scientific Reports

Article Title: Graphene oxide/Cu–MOF-based electrochemical immunosensor for the simultaneous detection of Mycoplasma pneumoniae and Legionella pneumophila antigens in water

doi: 10.1038/s41598-024-68231-y

Figure Lengend Snippet: The electrochemical characterization of the immobilization of the M. pneumoniae and L. pneumophila antigens using ( a,c ) cyclic voltammetry and ( b,d ) Differential pulse voltammetry in 1 mM [Fe(CN) 6 ] 3−/4− redox mediator in 0.01 M PBS (pH 7.4) electrolyte.

Article Snippet: Mouse anti- Mycoplasma pneumoniae P1 (6537), mouse anti- Legionella pneumophila (LN29) antibodies, Legionella pneumophila , mycoplasma pneumoniae antigens and interferents such as canine adenovirus 1 (CAV1), human metapneumovirus (hMPV) B, Influenza A (Cambodia) virus (IAV), Influenza B (Austria) virus (IBV), and respiratory syncytial virus B (RSVB) Lysate antigens were purchased from the Native Antigen company, United Kingdom.

Techniques:

Journal: Scientific Reports

Article Title: Graphene oxide/Cu–MOF-based electrochemical immunosensor for the simultaneous detection of Mycoplasma pneumoniae and Legionella pneumophila antigens in water

doi: 10.1038/s41598-024-68231-y

Figure Lengend Snippet: ( a ) The response of the dual immunosensor to 1 ng/mL of M. pneumoniae ( M. p. ), L. pneumophila ( L. p. ), and 10 ng/mL of CAV1, hMPV B, IAV, IBV, RSVB antigens. ( b ) Immunosensor responses for the concurrent detection of M. p. and L. p. both in a mixture and individually. The error bars represent the standard deviations of triplicate measurements.

Article Snippet: Mouse anti- Mycoplasma pneumoniae P1 (6537), mouse anti- Legionella pneumophila (LN29) antibodies, Legionella pneumophila , mycoplasma pneumoniae antigens and interferents such as canine adenovirus 1 (CAV1), human metapneumovirus (hMPV) B, Influenza A (Cambodia) virus (IAV), Influenza B (Austria) virus (IBV), and respiratory syncytial virus B (RSVB) Lysate antigens were purchased from the Native Antigen company, United Kingdom.

Techniques: